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KMID : 0368420040470040401
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Journal of Plant Biology
2004 Volume.47 No. 4 p.401 ~ p.406
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Molecular cloning of a cDNA encoding glutamine synthetase from root nodules ofElaeagnus umbellate
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Kim Ho-Bang
Kim Soo-Hyun
Lee Hyoung-Seok
Oh Chang-Jae
An Chung-Sun
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Abstract
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We analyzed a cDNA clone encoding cytosolic glutamine synthetase,EuNOD-GS1, isolated from a root nodule cDNA library ofElaeagnus umbellata. This clone has an insert size of 1359 bp and encodes a protein for 355 amino-acid residues, with a molecular weight of 39.2 kDa. Its expression is slightly higher in the root nodules than in the leaves or uninfected roots. Analysis of the deduced amino acid sequences and phytogeny revealed thatEuNOD-GS1 is clustered with cytosolic GS-α isoenzymes. Therefore, based on this and previous results, we propose that the main physiological role ofEuNOD-GS1 is the assimilation of ammonia from secondary and, in part, primary sources.
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KEYWORD
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cDNA, Elaeagnus umbellata, Frankia, glutamine synthetase, root nodule
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